Superresolution Microscopy @ Elyra 7

Superresolution microscopy enables insights into structures beyond the classical resolution limit. Since the introduction of Elyra 7, structured illumination microscopy (SIM) has been brought to a new level. Groundbreaking light efficiency empowers gentle superresolution imaging with incredibly high speed: to examine the fastest processes in living samples – in large fields of view, in 3D, over long time periods, and with multiple colors.
Elyra 7 combines Lattice SIM with single molecule localization microscopy (SMLM) for techniques such as PALM, dSTORM and PAINT.
This course will provide the skills to acquire Lattice SIM, PALM, and dSTORM data from specimens and will enable you to perform the appropriate processing. 

Topics of the course


Lattice SIM
  • Principle, acquisition and filtering
  • Versatility: SIM Mode, Leap Mode, Apotome Mode, Burst Mode
  • Camera alignment, channel alignment
  • PSF measurement
  • Processing


Single-molecule localization microscopy 

  • Basics and applications
  • Comparison: SMLM is more than PALM
  • Multichannel imaging
  • Grouping and working with fiducials
  • Activation strategies
  • 3D PALM


Learning Level




Profound knowledge of laser scanning microscopy



Elyra 7



ZEN SR black



January 31 to February 3, 2023
Registration until 2 weeks prior to the training



4 days



Training and Support Center Jena





Course Fees

Per person: 2880 € (plus VAT)



Max. 3


For additional information please send a request to courses .microscopy .de @zeiss .com.

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