
Microscopy Courses onsite at ZEISS Austria
Program 2023
Basics of Laser Scanning Microscopy

Course already took place
Course Language: English
Location: ZEISS Vienna
Laser Scanning Microscopy is a standard method in biomedical research. Excellent knowledge of its theory and application is a prerequisite for experimental success.
Key Learnings
- Take a deep dive in the basics of laser scanning microscopy (light, confocal beam path, image quality).
- During hands-on you will work intensively with the ZEISS LSM 900 on different topics (image acquisition and optimization, Z-stacks, time series).
- Get to know the newest advances like Airyscan jDCV and test them for yourself.
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Topics:
- Basics Light (diffraction, resolution)
- Beam path
- Image Quality (pixel, gain detectors, crosstalk)
Hands-On:
- Beam Path: creation of tracks, line/frame
- Image Optimization with: crosstalk-free, gain, speed, pixel
- Z-stack and time series
- LSM plus
- Tiles and positions
- Airyscan with jDCV
Correlative Microscopy & Image Analysis with ZEN

June 21, 2023 | 9:30 - 16:30
Course Language: English
Location: Materials Center Leoben
Key Learnings
- Experience the advantages and possibilities of the correlation from light and electron microscopy.
- We show you practically how to combine both methods. Test it for yourself in the lab of the Materials Center Leoben.
- Create sample-centric analysis results and reports based on different microscopy methods.
Fundamentals of Electron Microscopy

June 22, 2023 | 9:30 - 16:30
Course Language: English
Location: Materials Center Leoben
Key Learnings
- Master the fundamental principles of electron microscopy and comprehend their functionalities.
- Experience the different imaging and analytic detectors and how to apply them correctly.
- We show you practically how to acquire the best image with your system as well as a lot of valuable tips and tricks. You will be working on the ZEISS EVO MA 25.
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Topics:
- Basics of electron microscopy
- Practical operation of SEMs
- Settings of the electron beam vs. different issues
- Sample preparation
- Ideal microscopy settings for EDX measurements
- Support and development of solutions at SEM/EDX
- Hands-on examples
Training for the Imaging Software ZEN blue

September 14, 2023 | 9:30 - 16:30
Course Language: English
Location: ZEISS Vienna
Key Learnings
- In this training you will be introduced to the configuration of the ZEN blue Imaging Software and learn more about the Micro Tool Box.
- We will show you how to create multidimensional images using different software modules and focus strategies.
- You will work with the latest versions of ZEN (blue edition) and Axiocam digital microscopy cameras to learn how to perfectly acquire digital images and how to process and how to save them.
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Topics:
- Configuration of the Micro Tool Box.
- Introduction to the software-design, camera settings, microscope settings & acquisition.
- Scaling, scale bar and annotations
- Simple interactive measurements
- Selected ZEN Modules will be shown in detail (Z-Stack, Time Series, Tiles & Positions, extended depth of focus, autofocus, etc…
- Sytems: ZEISS Axio Observer, ZEISS Axio Imager und ZEISS Axio Zoom.
- You are welcome to bring your own samples.
Schedule
Day's Program
9:30 |
Welcome with Coffee |
---|---|
10:00 |
Start |
12:00 |
Lunch |
15:00 |
Coffee Break |
16:30 |
End |
Book Your Course
You can book each course individually. The courses are limited to 8 participants/course. A single course is charged at 600 EUR excl. VAT, special conditions of 900 EUR excl. VAT apply when booking two courses. More information can be found right here.
Locations
1. ZEISS Vienna
2. Materials Center Leoben
Your Trainers

Michael Zölffel
Michael studied biology, cell biology/protozoology in Göttingen, Berlin and Bristol and holds a PhD. He is a Fellow of the Royal Microscopical Society, Oxford .At ZEISS, Michael has now been working since 1996, first in Berlin, then in Göttingen and since 2018 in Jena. For him, microscopy is not only a profession, but also a vocation, and he has been practicing it in his spare time in addition to his professional activities for almost 45 years.

Sebastian Gliem
After the studies of neuroscience at the university of Göttingen Sebastian did his postdoc at the Max Planck Research School for Neurosciences. Since 2014 Sebastian is working at ZEISS in Jena as Senior Application Specialist and is responsible for the ZEISS Software ZEN Blue with the focus on Widefield and Automation. 2016 he was additionally Co-Facility Manager at the Harvard Center for Biological Imaging.

Christine Strasser
Christine Strasser studied biology and completed her PhD in Constance. During this time, she worked intensively with ZEISS confocal systems and conducted various training courses in the newly founded BioImaging Center. Since 2010 she has been working for ZEISS in Switzerland as an application specialist for confocal and lightsheet systems.

Bernhard Sartory
Bernhard Sartory has headed the SEM laboratory at the Materials Center Leoben since 2010. His international experience of more than 25 years includes various programs, contributions to renowned journals and participation in various international conferences in different fields.